Chen C H, Li B Y, Wan J T, Sun A, Leu J S, Chiang C P
Department of Dentistry, School of Dentistry, China Medical College, Taichung, Taiwan, ROC.
Proc Natl Sci Counc Repub China B. 2001 Apr;25(2):90-6.
This study used an immunohistochemical technique to assess the expression of epidermal growth factor (EGF) in 40 specimens of salivary adenoid cystic carcinoma (ACC), 7 specimens of labial glands adjacent to mucocele, and 5 specimens of normal submandibular glands. In normal submandibular glands, immunohistochemically detectable EGF was demonstrated in all ductal segments, including intercalated, striated, and excretory duct cells. No EGF positive staining was found in acinar compartments. including serous and mucous acinar cells. In degenerated labial glands adjacent to mucocele, no EGF staining was detected in the remaining acinar and ductal cells. In salivary ACCs, positive EGF immunostaining was observed in one of the 5 (20%) ACCs with a solid pattern and in 13 of the 35 (37.1%) ACCs with a tubular-cribriform pattern. The overall EGF expression rate in 40 salivary ACCs was 35%. Positive EGF staining was predominantly found in tubular structures in the tubular ACCs and in duct-like structures in large cribriform patterns or in the stroma of the cribriform ACCs. There was no significant correlation between EGF expression in salivary ACCs and any of the clinicopathological parameters including patient age and sex, cancer location, TNM status, clinical stage, histologic type, perivascular or perineural invasion, focal necrosis of tumor, and cellular atypia. We conclude that the duct segments of the normal submandibular gland are the sites of EGF synthesis and secretion. In degenerated labial glands adjacent to mucocele, EGF synthesis is completely inhibited. Furthermore, EGF is mainly biosynthesized in cells forming tubular or duct-like structures in tubular or cribriform salivary ACCs, and EGF may play a biologic role, particularly as a mitogen in salivary ACC growth.
本研究采用免疫组织化学技术，评估了40例涎腺腺样囊性癌（ACC）标本、7例黏液囊肿旁唇腺标本及5例正常下颌下腺标本中表皮生长因子（EGF）的表达情况。在正常下颌下腺中，免疫组织化学可检测到的EGF在所有导管段均有表达，包括闰管、纹状管和排泄管细胞。腺泡部分未发现EGF阳性染色，包括浆液性腺泡细胞和黏液性腺泡细胞。在黏液囊肿旁退变的唇腺中，剩余的腺泡和导管细胞未检测到EGF染色。在涎腺ACC中，5例（20%）实性型ACC中有1例观察到EGF免疫染色阳性，35例（37.1%）管状-筛状型ACC中有13例呈阳性。40例涎腺ACC的总体EGF表达率为35%。阳性EGF染色主要见于管状ACC的管状结构、大筛状型的导管样结构或筛状ACC的间质中。涎腺ACC中EGF表达与任何临床病理参数均无显著相关性，这些参数包括患者年龄和性别、肿瘤位置、TNM分期、临床分期、组织学类型、血管或神经侵犯、肿瘤局灶性坏死及细胞异型性。我们得出结论，正常下颌下腺的导管段是EGF合成和分泌的部位。在黏液囊肿旁退变的唇腺中，EGF合成被完全抑制。此外，EGF主要在管状或筛状涎腺ACC中形成管状或导管样结构的细胞中生物合成，EGF可能发挥生物学作用，尤其是作为涎腺ACC生长的促有丝分裂原。
