Ren X, Yang L, Liu J, Su D, You D, Liu C, Zhang K, Luo G, Mu Y, Yan G, Shen J
Key Laboratory of Molecular Enzymology and Engineering, Jilin University, Changchan, People's Republic of China.
Arch Biochem Biophys. 2001 Mar 15;387(2):250-6. doi: 10.1006/abbi.2000.2238.
Many diseases are associated with the overproduction of hydroperoxides that inflict cell damage. A novel cyclodextrin derivative, 6A,6B-diseleninic acid-6A',6B'-selenium bridged beta-cyclodextrin (6-diSeCD), was synthesized to be a functional mimic of glutathione peroxidase (GPX) that normally removes these hydroperoxides. The mimic had high catalytic GPX activity of 13.5 U/micromol, which is 13.6-fold higher than ebselen (PZ51), and was chemically and biologically stable in vitro. Antioxidant activity was studied by ferrous sulfate/ascorbate-induced mitochondria damage model system. These data show that the mimic has great antioxidant activity. Such mimics may result in better clinical therapies for diseases mediated by hydroperoxides.
许多疾病都与造成细胞损伤的氢过氧化物的过量产生有关。一种新型环糊精衍生物，6A,6B-二亚硒酸-6A',6B'-硒桥联β-环糊精（6-二硒环糊精，6-diSeCD），被合成出来作为谷胱甘肽过氧化物酶（GPX）的功能模拟物，GPX通常可清除这些氢过氧化物。该模拟物具有13.5 U/微摩尔的高催化GPX活性，比依布硒啉（PZ51）高13.6倍，并且在体外具有化学和生物学稳定性。通过硫酸亚铁/抗坏血酸诱导的线粒体损伤模型系统研究了抗氧化活性。这些数据表明该模拟物具有很强的抗氧化活性。此类模拟物可能会为氢过氧化物介导的疾病带来更好的临床治疗方法。
