Selamoglu Talas Zeliha, Ozdemir Ilknur, Yilmaz Ismet, Gok Yetkin, Orun Ibrahim
Department of Biology, Faculty of Arts and Science, Nigde University, Nigde, 51200 Turkey.
Exp Biol Med (Maywood). 2008 May;233(5):575-9. doi: 10.3181/0707-RM-191. Epub 2008 Mar 28.
DMBA (7,12-dimethylbenz[a]anthracene) is a polycyclic aromatic hydrocarbon (PAH) known to cause tumors in rats. Selenium is an essential element with physiological non-enzymatic antioxidant properties. Because of the health problems induced by many environmental pollutants, many efforts have been undertaken in evaluating the relative antioxidant potential of selenium and synthetic organoselenium compounds. In this study, adult female Wistar rats were treated with DMBA and the novel organoselenium compounds (1-isopropyl-3-methylbenzimidazole-2-selenone [SeI] and 1,3-di-p-methoxybenzylpyrimidine-2-selenone [SeII]) in the determined doses. The protective effects of novel synthetic organoselenium compounds (SeI and SeII) against DMBA-induced changes in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities and total glutathione (GSH) and malone-dialdehyde (MDA) levels of rat heart and brain were investigated. It was determined that SeI and SeII fully or partially restored enzyme activity. It was also found that lipid peroxidation was also decreased in SeI and SeII treated groups. Consequently, it was determined that novel synthetic organoselenium compounds (SeI and SeII) provided protection of antioxidant activity, and protection against lipid peroxidation measured as MDA in SeI and SeII treated groups was provided by novel synthesized organoselenium compounds. The ability of the organoselenium compounds to prevent oxidative damage induced by DMBA in rats was rationalized.
二甲基苯并蒽（7,12 - 二甲基苯并[a]蒽，DMBA）是一种已知可在大鼠体内引发肿瘤的多环芳烃（PAH）。硒是一种具有生理非酶抗氧化特性的必需元素。由于许多环境污染物会引发健康问题，人们已做出诸多努力来评估硒及合成有机硒化合物的相对抗氧化潜力。在本研究中，成年雌性Wistar大鼠接受了确定剂量的DMBA以及新型有机硒化合物（1 - 异丙基 - 3 - 甲基苯并咪唑 - 2 - 硒酮[SeI]和1,3 - 二对甲氧基苄基嘧啶 - 2 - 硒酮[SeII]）处理。研究了新型合成有机硒化合物（SeI和SeII）对DMBA诱导的大鼠心脏和大脑中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）活性以及总谷胱甘肽（GSH）和丙二醛（MDA）水平变化的保护作用。结果确定SeI和SeII能完全或部分恢复酶活性。还发现SeI和SeII处理组的脂质过氧化也有所降低。因此，确定新型合成有机硒化合物（SeI和SeII）可保护抗氧化活性，且SeI和SeII处理组中以MDA衡量的脂质过氧化保护作用是由新型合成有机硒化合物提供的。对有机硒化合物预防DMBA诱导的大鼠氧化损伤的能力进行了合理分析。
