Hu Z W, Thomas B R, Chernov A A
Universities Space Research Association, NASA/Marshall Space Flight Center, Huntsville, AL 35812, USA.
Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):840-6. doi: 10.1107/s0907444901005832. Epub 2001 May 25.
Double-axis multiple-crystal X-ray topography, rocking-curve measurements and triple-axis reciprocal-space mapping have been combined to characterize protein crystals using a laboratory source. Crystals of lysozyme and lysozyme crystals doped with acetylated lysozyme impurities were examined. It was shown that the incorporation of acetylated lysozyme into crystals of lysozyme induces mosaic domains that are responsible for the broadening and/or splitting of rocking curves and diffraction-space maps along the direction normal to the reciprocal-lattice vector, while the overall elastic lattice strain of the impurity-doped crystals does not appear to be appreciable in high angular resolution reciprocal-space maps. Multiple-crystal monochromatic X-ray topography, which is highly sensitive to lattice distortions, was used to reveal the spatial distribution of mosaic domains in crystals which correlates with the diffraction features in reciprocal space. Discussions of the influence of acetylated lysozyme on crystal perfection are given in terms of our observations.
利用实验室光源，将双轴多晶体X射线形貌术、摇摆曲线测量和三轴倒易空间映射相结合，以表征蛋白质晶体。对溶菌酶晶体以及掺杂有乙酰化溶菌酶杂质的溶菌酶晶体进行了检测。结果表明，将乙酰化溶菌酶掺入溶菌酶晶体中会诱导镶嵌畴，这些镶嵌畴导致摇摆曲线和衍射空间图沿垂直于倒易晶格矢量的方向变宽和/或分裂，而在高角分辨率倒易空间图中，掺杂杂质的晶体的整体弹性晶格应变似乎并不明显。对晶格畸变高度敏感的多晶体单色X射线形貌术被用于揭示晶体中镶嵌畴的空间分布，该分布与倒易空间中的衍射特征相关。根据我们的观察结果，对乙酰化溶菌酶对晶体完整性的影响进行了讨论。
