Boggon T J, Helliwell J R, Judge R A, Olczak A, Siddons D P, Snell E H, Stojanoff V
Chemistry Department, University of Manchester, Oxford Road, Manchester M13 9PL, England.
Acta Crystallogr D Biol Crystallogr. 2000 Jul;56(Pt 7):868-80. doi: 10.1107/s0907444900005837.
A comprehensive study of microgravity and ground-grown chicken egg-white lysozyme crystals is presented using synchrotron X-ray reciprocal-space mapping, topography techniques and diffraction resolution. Microgravity crystals displayed reduced intrinsic mosaicities on average, but no differences in terms of strain over their ground-grown counterparts. Topographic analysis revealed that in the microgravity case the majority of the crystal was contributing to the peak of the reflection at the appropriate Bragg angle. In the ground-control case only a small volume of the crystal contributed to the intensity at the diffraction peak. The techniques prove to be highly complementary, with the reciprocal-space mapping providing a quantitative measure of the crystal mosaicity and strain (or variation in lattice spacing) and the topography providing a qualitative overall assessment of the crystal in terms of its X-ray diffraction properties. Structural data collection was also carried out at the synchrotron.
利用同步加速器X射线倒易空间映射、形貌技术和衍射分辨率，对微重力环境下生长的和地面生长的鸡蛋白溶菌酶晶体进行了全面研究。微重力晶体平均显示出较低的固有镶嵌性，但与地面生长的对应晶体相比，在应变方面没有差异。形貌分析表明，在微重力情况下，大多数晶体在适当的布拉格角处对反射峰有贡献。在地面对照情况下，只有小部分晶体对衍射峰处的强度有贡献。这些技术被证明具有高度互补性，倒易空间映射提供了晶体镶嵌性和应变（或晶格间距变化）的定量测量，而形貌则根据晶体的X射线衍射特性对晶体进行定性的整体评估。结构数据收集也是在同步加速器上进行的。
