Fischer H, Stenling R, Rubio C, Lindblom A
Department of Molecular Medicine, Karolinska Institute, S 171 76 Stockholm, Sweden.
Carcinogenesis. 2001 Jun;22(6):875-8. doi: 10.1093/carcin/22.6.875.
Collagen is the major component of the interstitial extracellular matrix (ECM). ECM is known to play an active role in numerous biological processes such as cell shape, proliferation, migration, differentiation, apoptosis as well as carcinogenesis. We used mRNA differential display RT-PCR to study differentially expressed genes in tissue samples from 24 colorectal cancers and four normal colon epithelia. Twenty of the 24 tumours showed expression of a gene COL11A1, not expressed in the normal samples. This gene is not normally expressed in adult colon tissue, but was here found to be expressed in 27 out of a total of 34 (79%) colorectal carcinomas. An analysis of other collagens showed that COL5A2 was not expressed in normal colon but was co-expressed with COL11A1 in the tumours. Our results suggest that stromal expression of COL11A1 and COL5A2 is associated with malignancy in colorectal cancer.
胶原蛋白是细胞外间质（ECM）的主要成分。已知ECM在众多生物学过程中发挥积极作用，如细胞形态、增殖、迁移、分化、凋亡以及致癌作用。我们使用mRNA差异显示逆转录PCR技术研究了24例结直肠癌组织样本和4例正常结肠上皮组织中差异表达的基因。24个肿瘤中有20个显示出一种名为COL11A1的基因表达，而该基因在正常样本中未表达。该基因在成人结肠组织中通常不表达，但在此研究中发现，在总共34例结直肠癌中，有27例（79%）表达该基因。对其他胶原蛋白的分析表明，COL5A2在正常结肠中不表达，但在肿瘤中与COL11A1共表达。我们的结果表明，COL11A1和COL5A2的基质表达与结直肠癌的恶性程度相关。
