Jones N, Voskas D, Master Z, Sarao R, Jones J, Dumont D J
Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
EMBO Rep. 2001 May;2(5):438-45. doi: 10.1093/embo-reports/kve093.
Disruption of the signaling pathways mediated by the receptor tyrosine kinase Tek/Tie2 has shown that this receptor plays a pivotal role in vascularization of the developing embryo. In this report, we have utilized the tetracycline-responsive binary transgenic system to overcome the early lethal cardiovascular defects associated with the tekDeltasp null allele in order to investigate the role of Tek in later stages of vessel growth. We show for the first time in vivo that synchronized loss of tek expression correlates with rapid endothelial cell apoptosis in hemorrhagic regions of the embryo, demonstrating an ongoing requirement for Tek-mediated signal transduction in vascular maintenance.
由受体酪氨酸激酶Tek/Tie2介导的信号通路的破坏表明，该受体在发育中胚胎的血管形成中起关键作用。在本报告中，我们利用四环素反应性二元转基因系统来克服与tekDeltasp无效等位基因相关的早期致死性心血管缺陷，以研究Tek在血管生长后期阶段的作用。我们首次在体内表明，tek表达的同步缺失与胚胎出血区域中内皮细胞的快速凋亡相关，这表明在血管维持中持续需要Tek介导的信号转导。
