Volkov Y, Long A, McGrath S, Ni Eidhin D, Kelleher D
Dublin Molecular Medicine Centre, Trinity College, Dublin 8, Ireland.
Nat Immunol. 2001 Jun;2(6):508-14. doi: 10.1038/88700.
Crawling T cell locomotion in which activated lymphocyte function-associated antigen 1 (LFA-1) integrins participate is associated with translocation of the protein kinase C-beta (PKC-beta) isoenzyme to the microtubule cytoskeleton. In normal T cells and T lymphoma cell lines, this type of motility is accompanied by PKC-beta-sensitive cytoskeletal rearrangements and the formation of trailing cell extensions, which are supported by microtubules. Expression of PKC-beta(I) and enhanced green fluorescent protein (EGFP) in nonmotile PKC-beta-deficient T cells restored their locomotory behavior in response to a triggering stimulus delivered via LFA-1 and correlated directly with the degree of cell polarization. We have also shown that PKC-beta(I) is a component of the tubulin-enriched LFA-1-cytoskeletal complex assembled upon LFA-1 cross-linking. These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation.
活化淋巴细胞功能相关抗原1（LFA-1）整合素参与的爬行T细胞运动与蛋白激酶C-β（PKC-β）同工酶向微管细胞骨架的转位相关。在正常T细胞和T淋巴瘤细胞系中，这种运动类型伴随着PKC-β敏感的细胞骨架重排以及由微管支持的拖尾细胞延伸的形成。在无运动能力的PKC-β缺陷型T细胞中表达PKC-β（I）和增强型绿色荧光蛋白（EGFP）可恢复它们对通过LFA-1传递的触发刺激的运动行为，并且与细胞极化程度直接相关。我们还表明，PKC-β（I）是LFA-1交联后组装的富含微管蛋白的LFA-1-细胞骨架复合物的一个组成部分。这些观察结果可能在淋巴细胞外渗的晚期（整合素激活后）阶段具有生理等效物。
