过氧化氢酶在与氧化应激相关的不同人类疾病中的评估。

Catalase evaluation in different human diseases associated with oxidative stress.

作者信息

Al-Abrash A S, Al-Quobaili F A, Al-Akhras G N

机构信息

Department of Clinical Laboratory, Faculty of Pharmacy, Damascus University, Syria.

出版信息

Saudi Med J. 2000 Sep;21(9):826-30.

PMID:11376358

Abstract

OBJECTIVE

Catalase is an enzyme present in most of the aerobic cells, it protects them from oxidative stress by catalyzing the rapid decomposition of hydrogen peroxide (H2O2) in two types of reactions depending on its peroxidatic and catalatic activities. The aim of this study was to measure the erythrocytes catalase activity by a reliable method in normal subjects with different age categories, and patients whom suffer from different diseases associated with oxidative stress (inflammatory, tumor, diabetes, cardiovascular diseases, anemia and Wilson's disease).

METHODS

Erythrocytes catalase activity was measured, by peroxidatic method (Johansson-Borg method), in 210 apparently healthy subjects, (117 males and 93 females). The range of their ages was from 7 months to 65 years, and in 454 patients their ages ranged from 3 months to 74 years, whom suffer from the above mentioned diseases which resulted in oxidative stress. The comparison had been made between the Johansson-Borg and the UV catalase methods.

RESULTS

Strong correlation was found between the two methods, peroxidatic and catalatic (r=0.99, P<0.0001), but the catalase solutions were unstable when the temperature was raised. The normal range of catalase was found to be 2869+1039 u/g Hb. It was found that the catalase activity increased in the studied morbidity groups (eg. 188% in oxidative anemia). An accepted decrease 50% was noted in catalase activity when Vitamin E was administered to anemic patients suffering from oxidative stress.

CONCLUSION

There was an increase in catalase activity in all studied patients suffering from oxidative stress (cardiovascular diseases, diabetes, tumor, inflammation, dermatological diseases, anemia and Wilson's disease). The catalase activity was not affected by age, sex or the anticoagulant agent, which was used to collect the blood samples. It was found that the Vitamin E supplement decreased the catalase activity and improved the state of anemic oxidative stress patients.

摘要

目的

过氧化氢酶是一种存在于大多数需氧细胞中的酶，它通过催化过氧化氢（H2O2）根据其过氧化物酶和催化酶活性在两种反应中快速分解，从而保护细胞免受氧化应激。本研究的目的是采用一种可靠的方法，测量不同年龄组正常受试者以及患有与氧化应激相关的不同疾病（炎症、肿瘤、糖尿病、心血管疾病、贫血和威尔逊病）的患者的红细胞过氧化氢酶活性。

方法

采用过氧化物酶法（约翰松 - 博格法），对210名表面健康的受试者（117名男性和93名女性）测量红细胞过氧化氢酶活性。他们的年龄范围为7个月至65岁，对454名年龄范围为3个月至74岁、患有上述导致氧化应激疾病的患者也进行了测量。对约翰松 - 博格法和紫外过氧化氢酶法进行了比较。

结果

发现过氧化物酶法和催化酶法之间存在强相关性（r = 0.99，P < 0.0001），但温度升高时过氧化氢酶溶液不稳定。发现过氧化氢酶的正常范围为2869 + 1039 u/g Hb。发现在所研究的患病组中过氧化氢酶活性增加（例如，氧化型贫血中增加188%）。当向患有氧化应激的贫血患者给予维生素E时，过氧化氢酶活性出现公认的50%下降。