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突尼斯刺柏(Tetraclinis articulata (Vahl) Mast.)的精油:化学与生物学见解

Essential Oils of Tunisian Tetraclinis articulata (Vahl) Mast.: Chemical and Biological Insights.

作者信息

Ayed Amira, Caputo Lucia, De Feo Vincenzo, Nazzaro Filomena, Fratianni Florinda, Amri Ismail, Hamrouni Lamia, Mabrouk Yassine, Polito Flavio

机构信息

Laboratory of Biotechnology and Nuclear Technology, National Center for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet Technopark, Sidi Thabet, 2020, Tunisia.

Higher Institute of Biotechnology of Sidi Thabet (ISBST), University of Manouba, Sidi Thabet, 2020, Tunisia.

出版信息

Chem Biodivers. 2025 Jan;22(1):e202401618. doi: 10.1002/cbdv.202401618. Epub 2024 Oct 28.

Abstract

Tetraclinis articulata (Vahl) Mast. is native to the Mediterranean area and belongs to Cupressaceae family. The aim of this study were: i) to determine the chemical composition of essential oils (EOs) of T. articulata obtained from its stems, leaves, and cones using GC coupled to GC/MS; II) to evaluate their antioxidant activity using non enzymatic (DPPH, ABTS and FRAP) and enzymatic methods (catalase activity); III) to evaluated their anti-enzymatic activity on enzyme involved in metabolism and Central Nervous System using spectrophotometric assays. α-Pinene, limonene, and bornyl acetate were the main components of the three EOs. Moreover, the EO from cones showed the best antioxidant activity and was also to increase of catalase activity. All EOs were active against α-amylase in similar way; the EO leaves was more active against α-glucosidase and the EO from cones was more active against cholinesterase. The EOs demonstrated significant inhibition of the mature biofilm of Gram-negative and Gram-positive strains. This highlight the potential uses of T. articulata EOs in the fields of health and agriculture.

摘要

阿特拉斯柏(Tetraclinis articulata (Vahl) Mast.)原产于地中海地区,属于柏科。本研究的目的是:i)使用气相色谱联用气相色谱/质谱法(GC-MS)测定从阿特拉斯柏的茎、叶和球果中提取的挥发油(EOs)的化学成分;ii)使用非酶法(DPPH、ABTS和FRAP)和酶法(过氧化氢酶活性)评估其抗氧化活性;iii)使用分光光度法测定评估它们对参与新陈代谢和中枢神经系统的酶的抗酶活性。α-蒎烯、柠檬烯和乙酸龙脑酯是这三种挥发油的主要成分。此外,球果挥发油表现出最佳的抗氧化活性,并且还能提高过氧化氢酶活性。所有挥发油对α-淀粉酶的活性相似;叶挥发油对α-葡萄糖苷酶的活性更高,球果挥发油对胆碱酯酶的活性更高。这些挥发油对革兰氏阴性和革兰氏阳性菌株的成熟生物膜表现出显著抑制作用。这突出了阿特拉斯柏挥发油在健康和农业领域的潜在用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8192/11741151/32c667e5697d/CBDV-22-e202401618-g002.jpg

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