在黑腹果蝇成虫中鉴定出一种类DNA聚合酶β形式。
Identification of a DNA polymerase beta-like form in Drosophila melanogaster adult flies.
作者信息
Furia M, Polito L C, Locorotondo G, Grippo P
出版信息
Nucleic Acids Res. 1979 Jul 25;6(10):3399-410. doi: 10.1093/nar/6.10.3399.
DNA polymerase beta is widely distributed in the eukariotes. So far, few examples are known in which a DNA polymerase alpha -like form alone is reported. Surprisingly, DNA polymerase beta was not detected in Drosophila embryos, while it is present in the cells of multicellular species from sponge to mammals. In view of the relevance of Drosophila as a model biological system for studying the role of the various DNA metabolism enzymes in vivo we have reinvestigated the presence of the DNA polymerase beta-like form in Drosophila adult flies. Here we report the occurrence in Drosophila melanogaster adult flies of a DNA polymerase activity that, for its NEM(1) resistance, template specificity, sensitivity to ddTTP, sedimentation coefficient and nuclear localization can be classified as a beta-like form.
DNA聚合酶β广泛分布于真核生物中。到目前为止，仅报道了少数仅具有类似DNA聚合酶α形式的例子。令人惊讶的是，在果蝇胚胎中未检测到DNA聚合酶β，而在从海绵到哺乳动物的多细胞物种的细胞中却存在。鉴于果蝇作为研究各种DNA代谢酶在体内作用的模式生物系统的相关性，我们重新研究了果蝇成虫中类似DNA聚合酶β形式的存在情况。在此我们报道，在黑腹果蝇成虫中存在一种DNA聚合酶活性，就其对NEM（1）的抗性、模板特异性、对ddTTP的敏感性、沉降系数和核定位而言，可归类为类似β的形式。
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