Stafford D E, Stephanopoulos G
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Curr Opin Microbiol. 2001 Jun;4(3):336-40. doi: 10.1016/s1369-5274(00)00214-9.
Integration of the analytical framework and experimental tools of metabolic engineering with emerging technologies such as DNA microarrays and directed evolution stands to dramatically improve the approaches by which strain improvement and biocatalyst design are pursued in the future. Progress in genomics and applied molecular biology, together with increasing emphasis on renewable resource utilization for chemical production, has advanced metabolic engineering to the forefront of biotechnological interest.
将代谢工程的分析框架和实验工具与DNA微阵列和定向进化等新兴技术相结合，有望显著改进未来用于菌株改良和生物催化剂设计的方法。基因组学和应用分子生物学的进展，以及对利用可再生资源进行化学品生产的日益重视，已将代谢工程提升到生物技术关注的前沿。
