Gill Ryan T
Department of Chemical and Biological Engineering, UCB 424/ECCH120, University of Colorado, Boulder, CO 80304, USA.
Curr Opin Biotechnol. 2003 Oct;14(5):484-90. doi: 10.1016/s0958-1669(03)00116-2.
Inverse metabolic engineering (IME) is a powerful framework for engineering cellular phenotypes. Progress in this field has been limited by a lack of comprehensive methods for efficiently identifying the genetic basis of relevant phenotypes. Advances in genomics technologies, including DNA microarrays and gene sequencing, have dramatically improved our ability to relate changes in phenotype with associated changes in genotype. When applied in the context of IME, these tools should enable the integration of "evolutionary" and "direct" approaches to engineering cell physiology, which should improve our understanding of the complex interactions affecting the expression, evolution and engineering of traits in natural and industrial hosts.
逆向代谢工程（IME）是一种用于改造细胞表型的强大框架。该领域的进展一直受到缺乏有效识别相关表型遗传基础的综合方法的限制。包括DNA微阵列和基因测序在内的基因组技术的进步，极大地提高了我们将表型变化与基因型相关变化联系起来的能力。当在IME的背景下应用时，这些工具应能实现“进化”和“直接”方法在细胞生理学改造中的整合，这将增进我们对影响天然宿主和工业宿主中性状表达、进化及改造的复杂相互作用的理解。
