Tsujii K, Takagi T
J Biochem. 1975 Jan 1;77(1?):117-23.
Sodium n-octylbenzene-p-sulfonate was successfully used in place of sodium dodecyl sulfate in SDS-polyacrylamide gel electrophoresis. This modification of the usual technique made it possible to scan the polyacrylamide gel for the distribution of the surfactant by measurement of UV absorption. It was found that a band electrophoresed in advance of the complexes formed between protein polypeptides and the surfactant. The band could be observed even in the absence of protein polypeptide, and was ascribed to micelles derived from surfactant added in excess to the sample solution. The complexes were found to be detectable by scanning at 261 nm, usually with better sensitivity than by scanning at 280 nm. Thus, this modification of SDS-polyacrylamide gel electrophoresis is useful both to investigate what is going on in the gel during electrophoresis and to improve the sensitivity of detection by UV scanning of protein complexes.
正辛基苯磺酸钠成功地在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中替代了十二烷基硫酸钠。对常规技术的这种改进使得通过测量紫外吸收来扫描聚丙烯酰胺凝胶中表面活性剂的分布成为可能。结果发现，有一条带在蛋白质多肽与表面活性剂形成的复合物之前电泳。即使在没有蛋白质多肽的情况下也能观察到这条带，它被归因于样品溶液中过量添加的表面活性剂形成的胶束。发现通过在261nm处扫描可以检测到复合物，通常比在280nm处扫描具有更高的灵敏度。因此，这种对十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的改进，对于研究电泳过程中凝胶内发生的情况以及提高通过紫外扫描检测蛋白质复合物的灵敏度都很有用。
