Kubo K
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Anal Biochem. 1993 Sep;213(2):200-5. doi: 10.1006/abio.1993.1409.
In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at a concentration of 10-15% (v/v) to the sample-well gel supplemented to form the sample wells. This simple modification made the protein bands as sharp and straight as those in SDS-PAGE according to the Laemmli procedure. The linearity of the semilogarithmic plot of mobilities versus molecular mass was better than that obtained by Laemmli-type SDS-PAGE.
按照韦伯和奥斯本的方法进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）时，蛋白条带两端常常出现拖尾现象，致使条带变形，这种现象通常被称为“边缘拖尾”。通过向用于形成加样孔的加样孔凝胶中添加浓度为10 - 15%（v/v）的甘油，可消除这一现象。这一简单的改进使得蛋白条带与按照莱姆利方法进行的SDS-PAGE中的条带一样清晰笔直。迁移率与分子量的半对数图的线性比莱姆利型SDS-PAGE得到的更好。
