Calzada J E, Nieto A, López-Nevot M A, Martín J
Instituto de Parasitología y Biomedicina "López Neyra", CSIC, Granada, Spain.
Tissue Antigens. 2001 Apr;57(4):353-7. doi: 10.1034/j.1399-0039.2001.057004353.x.
Genetic analysis in mice and humans have established the key role of the human natural resistance-associated macrophage protein 1 (NRAMP1) in resistance to intracellular infections. In the present study we investigated whether four NRAMP1 polymorphisms (5'(GT)n, -236 C-->T, D543N, and 3'UTR deletion) were important in determining the susceptibility to Trypanosoma cruzi infections as well as in the development of chagasic cardiac disease. Genotyping for these variants was assessed in 83 seropositive (asymptomatic, n=51, cardiomyopathic, n=32) and 85 seronegative individuals from a Peruvian population where T. cruzi is endemic. No statistically significant differences either between patients and controls or between asymptomatic and cardiomyopathic individuals were observed with respect to NRAMP1 variants. Our data suggest that the NRAMP1 genetic polymorphism analysed do not play a major role in the pathogenesis of T. cruzi infection in this Peruvian sample.
对小鼠和人类的基因分析已证实人类天然抗性相关巨噬细胞蛋白1（NRAMP1）在抵抗细胞内感染中起关键作用。在本研究中，我们调查了四种NRAMP1多态性（5'（GT）n、-236 C→T、D543N和3'UTR缺失）在确定克氏锥虫感染易感性以及恰加斯心肌病发展过程中是否重要。在来自克氏锥虫流行的秘鲁人群的83名血清阳性个体（无症状，n = 51；心肌病患者，n = 32）和85名血清阴性个体中对这些变异进行基因分型。就NRAMP1变异而言，在患者与对照之间或无症状个体与心肌病患者之间均未观察到统计学上的显著差异。我们的数据表明，在这个秘鲁样本中，所分析的NRAMP1基因多态性在克氏锥虫感染的发病机制中不发挥主要作用。
