腈水解酶超家族：分类、结构与功能。

Structural Biology and Bioinformatics Program, Kimmel Cancer Center, 233 S Tenth Street, Philadelphia, PA 19107, USA.

Genome Biol. 2001;2(1):REVIEWS0001. doi: 10.1186/gb-2001-2-1-reviews0001. Epub 2001 Jan 15.

The nitrilase superfamily consists of thiol enzymes involved in natural product biosynthesis and post-translational modification in plants, animals, fungi and certain prokaryotes. On the basis of sequence similarity and the presence of additional domains, the superfamily can be classified into 13 branches, nine of which have known or deduced specificity for specific nitrile- or amide-hydrolysis or amide-condensation reactions. Genetic and biochemical analysis of the family members and their associated domains assists in predicting the localization, specificity and cell biology of hundreds of uncharacterized protein sequences.

腈水解酶超家族由参与植物、动物、真菌和某些原核生物中天然产物生物合成和翻译后修饰的硫醇酶组成。根据序列相似性和其他结构域的存在情况，该超家族可分为13个分支，其中9个分支对特定的腈水解或酰胺水解或酰胺缩合反应具有已知或推测的特异性。对家族成员及其相关结构域进行遗传和生化分析，有助于预测数百个未表征蛋白质序列的定位、特异性和细胞生物学特性。