Taylor R B, Reid R G, Low A S
School of Pharmacy, Robert Gordon University, Schoolhill, Aberdeen, UK.
J Chromatogr A. 2001 May 4;916(1-2):201-6. doi: 10.1016/s0021-9673(00)01035-9.
The method of applying large sample volumes in micellar electrokinetic chromatography termed sweeping is applied to determine the conservative limits of detection of some basic drugs in plasma and urine. The biguanides proguanil, 4-chlorophenylbiguanide and cycloguanil are used as models of basic drugs and the limits of detection obtained compared with those previously reported for capillary zone electrophoresis using field-amplified sample injection (FASI) and also by LC using off-line preconcentration. It is found that the sweeping method can be applied to extracts of such biological matrices. The limits of detection obtained by sweeping are improved over FASI for plasma but not for urine and the limits of detection are higher than those reported for LC, for these compounds.
在胶束电动色谱中应用大体积进样的“推扫”方法来测定血浆和尿液中某些碱性药物的保守检测限。将双胍类药物氯胍、4-氯苯双胍和环氯胍用作碱性药物的模型，并将所得检测限与先前报道的使用场放大进样（FASI）的毛细管区带电泳以及使用离线预浓缩的液相色谱法的检测限进行比较。结果发现，推扫方法可应用于此类生物基质的提取物。对于血浆，推扫法获得的检测限优于FASI，但对于尿液则不然，并且对于这些化合物，推扫法的检测限高于液相色谱法报道的检测限。
