大鼠快速眼动睡眠剥夺后，脑桥和海马突触体中毒蕈碱M2受体减少。

Decrease in muscarinic M2 receptors from synaptosomes in the pons and hippocampus after REM sleep deprivation in rats.

作者信息

Salín-Pascual R J, Díaz-Muñoz M, Rivera-Valerdi L, Ortiz-López L, Blanco-Centurión C

机构信息

Departamento de Fisiología, Universidad Nacional Autonoma de Mexico, México, D.F. 04510, México.

出版信息

Sleep Res Online. 1998;1(1):19-23.

PMID:11382853

Abstract

The effects of both REM sleep deprivation and its recovery on pontine and hippocampus muscarinic M2 receptors were investigated in synaptosomes using [3H]-AF-DX 384 as a ligand. Animals were divided into three groups: REM sleep deprivation group (small platforms 6.5 cm of diameter); stress group (large platforms 14 cm of diameter) and cage control group. In a second experiment REM sleep-deprived animals were allowed 48 h of recovery. REM sleep-deprived rats showed a reduction in M2 receptors compared with both intact and stress groups. Changes in M2 receptors were also observed after 48 h of recovery from REM sleep deprivation only in hippocampus. The enhancement of acetylcholine release during both REM sleep deprivation and recovery could explain the present findings.

摘要

使用[3H]-AF-DX 384作为配体，在突触体中研究了快速眼动（REM）睡眠剥夺及其恢复对脑桥和海马毒蕈碱M2受体的影响。动物被分为三组：REM睡眠剥夺组（直径6.5厘米的小平台）；应激组（直径14厘米的大平台）和笼养对照组。在第二个实验中，让REM睡眠剥夺的动物恢复48小时。与完整组和应激组相比，REM睡眠剥夺的大鼠M2受体减少。仅在海马中，从REM睡眠剥夺恢复48小时后也观察到M2受体的变化。REM睡眠剥夺和恢复期间乙酰胆碱释放的增强可以解释目前的研究结果。