Wolff Samuel C, Hruska Zuzana, Nguyen Lieuko, Dohanich Gary P
Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA.
Eur J Pharmacol. 2008 Jul 7;588(2-3):248-50. doi: 10.1016/j.ejphar.2008.04.002. Epub 2008 Apr 9.
Asymmetries in muscarinic receptor binding were investigated in the hippocampus of female rats by in vitro autoradiography. Coronal sections from 18 brains were incubated with the muscarinic receptor antagonist [3H]quinuclidinyl benzilate, the muscarinic M1 receptor antagonist [3H]pirenzepine, or the muscarinic M2 receptor antagonist [3H]AF-DX 384. Binding of these radioligands was higher on the right than the left side of CA1, CA3, and dentate gyrus in almost every brain confirming hemispheric asymmetry at the neurochemical level. The ovarian hormone, estradiol, did not alter the asymmetry in muscarinic binding. Neurochemical asymmetries within hippocampal subfields may have implications for physiological and behavioral functions.
通过体外放射自显影术研究了雌性大鼠海马中毒蕈碱受体结合的不对称性。用毒蕈碱受体拮抗剂[3H]喹核醇基苯甲酸酯、毒蕈碱M1受体拮抗剂[3H]哌仑西平或毒蕈碱M2受体拮抗剂[3H]AF-DX 384对18个大脑的冠状切片进行孵育。在几乎每一个大脑中，这些放射性配体在CA1、CA3和齿状回右侧的结合都高于左侧，证实了在神经化学水平上的半球不对称性。卵巢激素雌二醇并未改变毒蕈碱结合的不对称性。海马亚区内的神经化学不对称可能对生理和行为功能有影响。
