丝裂霉素C可减少兔准分子激光屈光性角膜切削术后的 haze 形成。

Mitomycin C reduces haze formation in rabbits after excimer laser photorefractive keratectomy.

作者信息

Xu H, Liu S, Xia X, Huang P, Wang P, Wu X

机构信息

Department of Ophthalmology, Xiangya Hospital, Hunan Medical University, Changsha, China.

出版信息

J Refract Surg. 2001 May-Jun;17(3):342-9. doi: 10.3928/1081-597X-20010501-08.

DOI:10.3928/1081-597X-20010501-08

PMID:11383766

Abstract

PURPOSE

To investigate the effects of mitomycin C on haze after photorefractive keratectomy (PRK).

METHODS

Twenty of 24 rabbits underwent bilateral 193-nm excimer laser PRK to correct -10.00 D of myopia; the remaining four rabbits were not operated (no PRK group). The right eyes of the 20 rabbits were treated with 0.02% mitomycin C during surgery (PRK+MMC group) and the left eyes did not receive 0.02% mitomycin C (PRK alone group). Clinical and histopathologic examinations were performed.

RESULTS

The most severe haze in the PRK alone group after PRK reached grade 3; the PRK+MMC group did not exceed grade 1 haze. Statistically significant differences were found between the PRK+MMC and PRK alone groups from week 2 to week 26 after treatment (P<.01). Epithelial thickening appeared for 26 weeks in both PRK groups; no statistically significant differences were found between the two PRK groups (P>.05). A marked reduction of keratocytes in the anterior stroma of the PRK+MMC group was observed. At week 1, 2, and 4 after PRK, keratocytes of the PRK+MMC group were only 3.1+/-2.6, 6.8+/-4.7, and 12.4+/-5.7 keratocytes x 10(4)/microm2, respectively, while those of the PRK alone group were 41.2+/-80, 42.3+/-7.8, and 40.0+/-3.3 keratocytes x 10(4)/microm2, respectively. There were statistically significant differences between the two groups (P<.001).

CONCLUSION

A single intraoperative application of topical mitomycin C during PRK in rabbits reduced corneal haze by inhibiting the proliferation of keratocytes.

摘要

目的

研究丝裂霉素C对准分子激光原位角膜磨镶术（PRK）后角膜雾状混浊的影响。

方法

24只兔子中的20只接受双侧193纳米准分子激光PRK以矫正-10.00 D近视；其余4只兔子未手术（非PRK组）。20只兔子的右眼在手术期间用0.02%丝裂霉素C治疗（PRK+MMC组），左眼未接受0.02%丝裂霉素C（单纯PRK组）。进行了临床和组织病理学检查。

结果

单纯PRK组PRK后最严重的角膜雾状混浊达到3级；PRK+MMC组未超过1级角膜雾状混浊。治疗后第2周和第26周，PRK+MMC组和单纯PRK组之间存在统计学显著差异（P<0.01）。两个PRK组的上皮增厚均出现26周；两个PRK组之间未发现统计学显著差异（P>0.05）。观察到PRK+MMC组前基质中的角膜细胞明显减少。PRK后第1周、第2周和第4周，PRK+MMC组的角膜细胞分别仅为3.1±2.6、6.8±4.7和12.4±5.7个角膜细胞×10(4)/平方微米，而单纯PRK组分别为41.2±80、42.3±7.8和40.0±3.3个角膜细胞×10(4)/平方微米。两组之间存在统计学显著差异（P<0.001）。