Shishkin S S, Kitavtsev B A, Debov S S
Vopr Med Khim. 1975 Mar-Apr;21(2):137-42.
Possibility was investigated to prepare polysomes from microsomal fraction of rat sceletal muscles. Centrifugation in sucrose gradient showed that approximately 60 percent of UV-absorbing material in the preparation obtained was in polysomic region of the gradient. Mild treatment with pancreatic ribonuclease led to disintegration of polysomes and respective increase in content of monosomic component. The use of combined gradient permitted to obtain two fractions of polysomes, one of which was readily sedimented by centrifugation. This fraction contained significant amount of protein and incorporated relatively low quantity of 32-P within 2 hrs after administration of the label in vivo. The data obtained suggest that the fraction of polysomes carried out the biosynthesis of myosin.
研究了从大鼠骨骼肌微粒体部分制备多核糖体的可能性。蔗糖梯度离心显示，所获得制剂中约60%的紫外线吸收物质位于梯度的多核糖体区域。用胰核糖核酸酶进行温和处理导致多核糖体解体，单体成分含量相应增加。使用组合梯度可获得两部分多核糖体，其中一部分很容易通过离心沉淀。该部分含有大量蛋白质，在体内给予标记物后2小时内掺入的32-P量相对较低。所获得的数据表明，该部分多核糖体进行了肌球蛋白的生物合成。
