Kitavtsev B A, Shishkin S S
Vopr Med Khim. 1976 Nov-Dec;22(6):815-9.
Alterations in properties of RNP-particles from rat sceletal muscles on storage of the preparations in buffer solutions at various pH were analyzed by means of centrifugation in sucrose gradients. Within 3 days of incubation at 20 the distribution of RNP-particles in the sucrose gradient was changed relatively slightly; which was due to the low activity of endogenous ribosomal RNAase. The fraction of the UV-absorbing material in polysomes was gradually decreased on storage and the fraction of subunits was increased respectively. The most distinct alterations were observed on incubation of the preparations at pH 6.0; the RNP-particles were apparently destructed after acidification of the medium.
通过在蔗糖梯度中离心，分析了大鼠骨骼肌的核糖核蛋白（RNP）颗粒制剂在不同pH值的缓冲溶液中储存时其性质的变化。在20℃孵育3天内，RNP颗粒在蔗糖梯度中的分布变化相对较小；这是由于内源性核糖体核糖核酸酶活性较低。储存时，多核糖体中紫外线吸收物质的比例逐渐降低，亚基的比例则相应增加。在pH 6.0条件下孵育制剂时观察到最明显的变化；培养基酸化后，RNP颗粒明显被破坏。
