Smith G C, Wu W X, Nathanielsz P W
Laboratory for Pregnancy and Newborn Research, Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Mol Hum Reprod. 2001 Jun;7(6):587-94. doi: 10.1093/molehr/7.6.587.
Myometrium (upper and lower segment), cervix, chorion and decidua were obtained at the time of Caesarean hysterectomy in 15 baboons, 10 at varying gestational ages prior to the onset of labour in the last trimester of pregnancy, and five in spontaneous labour at term. Expression of currently recognized human lipoxygenase (LOX) genes was determined using Northern blot analysis relative to each of three housekeeping genes. Signals of similar size to human 5-LOX and human platelet 12-LOX genes were detected in all tissues. Expression of 5-LOX mRNA in the cervix decreased with advancing gestational age. In decidua, expression of 5-LOX mRNA was higher in tissues from animals in labour (compared to those not in labour), whereas in chorion, its expression was lower in tissues from animals in labour. Expression of the platelet 12-LOX gene decreased in chorion with advancing gestational age, and in cervix was lower in tissues from animals in labour. We postulate that the variation in expression of LOX genes may play a role in the onset or promotion of parturition in the baboon.
在15只狒狒进行剖宫产子宫切除手术时获取子宫肌层（上段和下段）、子宫颈、绒毛膜和蜕膜，其中10只处于妊娠晚期分娩开始前不同孕周，5只处于足月自然分娩。使用Northern印迹分析相对于三个管家基因中的每一个来确定当前公认的人类脂氧合酶（LOX）基因的表达。在所有组织中均检测到与人类5-LOX和人类血小板12-LOX基因大小相似的信号。随着孕周增加，子宫颈中5-LOX mRNA的表达降低。在蜕膜中，分娩动物组织中5-LOX mRNA的表达高于未分娩动物（相比之下），而在绒毛膜中，分娩动物组织中其表达较低。随着孕周增加，绒毛膜中血小板12-LOX基因的表达降低，并且在子宫颈中，分娩动物组织中的表达较低。我们推测LOX基因表达的变化可能在狒狒分娩的发动或促进中起作用。
