Bigiani A
Dipartimento di Scienze Biomediche, Universitá di Modena e Reggio Emilia, Italy.
Neuroreport. 2001 May 25;12(7):1315-21. doi: 10.1097/00001756-200105250-00006.
Sodium ions occurring in food are thought to be detected, at least in part, through specific amiloride-sensitive, sodium channels (ASSCs) localized in taste receptor cells. Cells within taste buds are morphologically heterogeneous, and include taste receptor cells and other cells that could perform a support or even transduction role. It is not known whether subsets of the taste bud cells express ASSCs, and whether the properties of these channels are similar. By applying the patch-clamp technique to morphologically distinct cells, the supporting wing cells, isolated from the frog taste disk, I have found functional ASSCs that are moderately sensitive to amiloride (Ki 3-4 microM) and which are distinctly lower in affinity for amiloride than reported frog taste receptor cells (Ki 0.2 microM). These results support the hypotheses of the existence of distinct, functional ASSCs in different cell morphotypes, at least in frog taste organs.
人们认为，食物中的钠离子至少部分是通过位于味觉受体细胞中的特定阿米洛利敏感钠通道（ASSCs）来检测的。味蕾中的细胞在形态上是异质的，包括味觉受体细胞和其他可能起支持甚至转导作用的细胞。目前尚不清楚味蕾细胞亚群是否表达ASSCs，以及这些通道的特性是否相似。通过将膜片钳技术应用于形态不同的细胞，即从青蛙味觉盘分离出的支持性翼状细胞，我发现了对阿米洛利中度敏感（Ki为3 - 4 microM）且对阿米洛利的亲和力明显低于已报道的青蛙味觉受体细胞（Ki为0.2 microM）的功能性ASSCs。这些结果支持了至少在青蛙味觉器官中，不同细胞形态类型中存在独特功能性ASSCs这一假说。
