来自不同来源的伊氏锥虫分离株中RoTat 1.2可变抗原型的总体表达情况。

General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin.

作者信息

Verloo D, Magnus E, Büscher P

机构信息

Department of Parasitology, Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerpen, Belgium.

出版信息

Vet Parasitol. 2001 Jun 12;97(3):183-9. doi: 10.1016/s0304-4017(01)00412-5.

DOI:10.1016/s0304-4017(01)00412-5

PMID:11390070

Abstract

The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we checked its expression in 10 different T. evansi stocks and clones from different parts of the world. Cryostabilates were injected into mice and the trypanosomes of the first peak parasitaemia were screened for the presence of RoTat 1.2 by VAT specific immunofluorescence. To monitor the appearance of RoTat 1.2 specific antibodies during infection, rabbits were infected and serologically tested at different time intervals with VAT specific immune trypanolysis, CATT/T. evansi, LATEX/T. evansi and ELISA/T. evansi. Test results confirm the predominant character of RoTat 1.2.

摘要

伊氏锥虫RoTat 1.2可变抗原型（VAT）的可变表面糖蛋白在伊氏锥虫不同抗体检测试验中用作抗原。为了获取更多关于RoTat 1.2的主要特性及其在抗体检测试验中的诊断潜力的信息，我们检查了来自世界各地的10种不同伊氏锥虫虫株和克隆中RoTat 1.2的表达情况。将冷冻保存物注射到小鼠体内，通过VAT特异性免疫荧光筛选首次出现寄生虫血症高峰时的锥虫是否存在RoTat 1.2。为监测感染期间RoTat 1.2特异性抗体的出现情况，用VAT特异性免疫锥虫溶解试验、CATT/伊氏锥虫试验、乳胶/伊氏锥虫试验和ELISA/伊氏锥虫试验，在不同时间间隔对感染的兔子进行血清学检测。试验结果证实了RoTat 1.2的主要特性。