Thompson N A, Haefliger J A, Senn A, Tawadros T, Magara F, Ledermann B, Nicod P, Waeber G
Department of Internal Medicine and Institute of Cellular Biology and Morphology and the Reproductive Medicine Unit, CHUV-University Hospital, 1011 Lausanne, Switzerland.
J Biol Chem. 2001 Jul 27;276(30):27745-8. doi: 10.1074/jbc.C100222200. Epub 2001 Jun 4.
Islet-brain1/JNK-interacting protein-1 (IB1/JIP-1) is a scaffold protein that organizes the JNK, MKK7, and MLK1 to allow signaling specificity. Targeted disruption of the gene MAPK8IP1 encoding IB1/JIP-1 in mice led to embryonic death prior to blastocyst implantation. In culture, no IB1/JIP-1(-/-) embryos were identified indicating that accelerated cell death occurred during the first cell cycles. IB1/JIP-1 expression was detected in unfertilized oocytes, in spermatozoa, and in different stages of embryo development. Thus, despite the maternal and paternal transmission of the IB1/JIP-1 protein, early transcription of the MAPK8IP1 gene is required for the survival of the fertilized oocytes.
胰岛-脑1/JNK相互作用蛋白-1（IB1/JIP-1）是一种支架蛋白，它组织JNK、MKK7和MLK1以实现信号特异性。在小鼠中靶向破坏编码IB1/JIP-1的基因MAPK8IP1会导致在胚泡植入前胚胎死亡。在培养中，未鉴定出IB1/JIP-1(-/-)胚胎，这表明在最初的细胞周期中发生了加速的细胞死亡。在未受精卵母细胞、精子以及胚胎发育的不同阶段均检测到IB1/JIP-1的表达。因此，尽管IB1/JIP-1蛋白存在母系和父系传递，但MAPK8IP1基因的早期转录对于受精卵母细胞的存活是必需的。
