采用非侵入性方法测量人类皮肤中皮质类固醇诱导的萎缩和屏障损伤。
Corticosteroid-induced atrophy and barrier impairment measured by non-invasive methods in human skin.
作者信息
Kolbe L, Kligman A M, Schreiner V, Stoudemayer T
机构信息
S.K.I.N. Inc., Conshohocken, PA, USA.
出版信息
Skin Res Technol. 2001 May;7(2):73-7. doi: 10.1034/j.1600-0846.2001.70203.x.
BACKGROUND/AIMS: Atrophy is a distressing side effect of potent corticosteroids. After open application of a high potency steroid, we monitored atrophogenicity by a variety of non-invasive methods.
METHODS
Volar forearms were treated twice daily for 3 or 4 weeks, with clobetasol propionate cream (Temovate). The following methods were used: 1) confocal microscopy, 2) transepidermal water loss (TEWL), 3) dimethyl sulfoxide whealing, 4) sodium hydroxide erosions, 5) analysis of stratum corneum lipids, and 6) B-scan ultrasound.
RESULTS
Confocal microscopy revealed thinning of the epidermis, decreased microvasculature and decreased size of keratinocytes. Evaporimetry demonstrated transepidermal water loss. Whealing to dimethyl sulfoxide was enhanced. Sodium hydroxide erosions formed more quickly. The amount of ceramides, cholesterol, and free fatty acids was reduced. Ultrasound showed thinning of the dermis.
CONCLUSION
Non-invasive methods are very useful for quantifying the atrophogenicity of topical corticosteroids.
背景/目的：萎缩是强效皮质类固醇令人苦恼的副作用。在开放性应用高效类固醇后，我们通过多种非侵入性方法监测致萎缩性。
方法
用丙酸氯倍他索乳膏（特美肤）对前臂掌侧进行每日两次、为期3或4周的治疗。采用了以下方法：1）共聚焦显微镜检查；2）经表皮水分流失（TEWL）；3）二甲亚砜风团试验；4）氢氧化钠侵蚀试验；5）角质层脂质分析；6）B型超声扫描。
结果
共聚焦显微镜检查显示表皮变薄、微血管减少以及角质形成细胞大小减小。蒸发测定法表明经表皮水分流失。对二甲亚砜的风团反应增强。氢氧化钠侵蚀形成更快。神经酰胺、胆固醇和游离脂肪酸的量减少。超声显示真皮变薄。
结论
非侵入性方法对于量化外用皮质类固醇的致萎缩性非常有用。
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