Pappa-Louisi A, Papageorgiou A, Zitrou A, Sotiropoulos S, Georgarakis E, Zougrou F
Department of Chemistry, Aristotle University of Thessaloniki, Greece.
J Chromatogr B Biomed Sci Appl. 2001 May 5;755(1-2):57-64. doi: 10.1016/s0378-4347(00)00614-9.
The optimal conditions of the amperometric detection of the macrolide antibiotics clarithromycin and roxithromycin were found by cyclic voltammetric studies and HPLC-electrochemical detection responses obtained in different temperatures (25.5-60 degrees C) and different but almost isoelutropic binary, ternary and quaternary mixtures of aqueous buffer (pH 7), methanol, acetonitrile and isopropanol. These conditions were also proved to be applicable for the quantitative detection of clarithromycin in human plasma using roxithromycin as an internal standard and vice versa. It was demonstrated that increased attention has to be paid to eluent composition and column temperature to ensure sensitive and reproducible electrochemical responses as well as regularly shaped peaks for both macrolides tested.
通过循环伏安研究以及在不同温度（25.5 - 60摄氏度）和不同但几乎等度洗脱的二元、三元和四元混合物（含水缓冲液（pH 7）、甲醇、乙腈和异丙醇）中获得的高效液相色谱 - 电化学检测响应，找到了测定大环内酯类抗生素克拉霉素和罗红霉素的安培检测的最佳条件。这些条件也被证明适用于以罗红霉素为内标定量检测人血浆中的克拉霉素，反之亦然。结果表明，必须更加关注洗脱液组成和柱温，以确保对所测试的两种大环内酯类药物都能获得灵敏且可重现的电化学响应以及形状规则的峰。
