Hedenmo M, Eriksson B M
Astra Hässle AB, Mölndal, Sweden.
J Chromatogr A. 1995 Feb 10;692(1-2):161-6. doi: 10.1016/0021-9673(94)00610-l.
A liquid chromatographic method for the determination of the macrolide antibiotics, roxithromycin and clarithromycin, in plasma is described. The method is fully automated, employing on-line solid-phase extraction for sample clean-up, using the Prospekt unit. Plasma samples, mixed with internal standard, were injected onto exchangeable CN cartridges. After washing, the compounds were eluted and transferred to a C18 analytical column for separation and electrochemical detection. Clarithromycin was used as internal standard when assaying roxithromycin and vice versa. The recovery of the solid-phase extraction method was 90% and higher, and the relative standard deviation was about 3%. The limit of quantitation was 0.5 mumol/l when 25 microliters of plasma was injected. Comparison with a liquid-liquid extraction method for sample clean-up showed good agreement.
本文描述了一种用于测定血浆中大分子内酯抗生素罗红霉素和克拉霉素的液相色谱方法。该方法完全自动化，采用在线固相萃取进行样品净化，使用Prospekt装置。将血浆样品与内标混合后注入可更换的CN柱。洗涤后，洗脱化合物并转移至C18分析柱进行分离和电化学检测。测定罗红霉素时以克拉霉素为内标，反之亦然。固相萃取法的回收率在90%及以上，相对标准偏差约为3%。当进样25微升血浆时，定量限为0.5微摩尔/升。与液-液萃取法进行样品净化的比较显示出良好的一致性。
