Wu X D, Du L N, Wu G C, Cao X D
Institute of Acupuncture Research (WHO Collaborating Center for Traditional Medicine), Medical School of Fudan University, Shanghai, People's Republic of China.
Acupunct Electrother Res. 2001;26(1-2):1-9. doi: 10.3727/036012901816356063.
The effect of electroacupuncture (EA) on the blood-brain barrier (BBB) after cerebral ischemia-reperfusion (I-R) was investigated in rats. The dye Evans Blue (EB) was used as a tracer for assessing the disruption of BBB. Fluorescence quantification of EB was performed to explore the temporal pattern of permeability of BBB after the cerebral I-R with a fluorescence spectrophotometer (HITACHI 650-60). Furthermore, the morphology of BBB opening was detected under confocal laser scanning microscopy system. It was found that the BBB opening after cerebral I-R was biphasic. A rare scattered extravasation of EB was detected 2 hours after cerebral I-R. The EB extravasation reached its first peak at 6 h then decreased at 24 h and increased again at the time-point of 48h after cerebral I-R. EA can attenuate the disruption of BBB after cerebral I-R. EA could not only limit the area of extravasation of EB, but also reduce the concentration of extravasation of EB in the rat brain after cerebral I-R. The results indicated that one of the mechanisms of curative effect of EA on the cerebral ischemia might be due to its function of protecting the integrity of BBB.
在大鼠中研究了电针（EA）对脑缺血再灌注（I-R）后血脑屏障（BBB）的影响。使用伊文思蓝（EB）染料作为评估血脑屏障破坏的示踪剂。用荧光分光光度计（日立650-60）对EB进行荧光定量，以探索脑I-R后血脑屏障通透性的时间模式。此外，在共聚焦激光扫描显微镜系统下检测血脑屏障开放的形态。发现脑I-R后的血脑屏障开放是双相的。脑I-R后2小时检测到罕见的散在EB外渗。EB外渗在6小时达到第一个峰值，然后在24小时下降，并在脑I-R后48小时再次增加。电针可以减轻脑I-R后血脑屏障的破坏。电针不仅可以限制EB外渗的面积，还可以降低脑I-R后大鼠脑中EB外渗的浓度。结果表明，电针治疗脑缺血疗效的机制之一可能是由于其保护血脑屏障完整性的功能。
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