Ye Tao, Zhu Luwen, Tang Qiang, Li Hongyu, Wu Xiaojun, Jiang Yunfei
Heilongjiang University of CM, Harbin 150040, China.
the Second Hospital Affiliated to Heilongjiang University of CM, Harbin 150001.
Zhongguo Zhen Jiu. 2017 Oct 12;37(10):1093-7. doi: 10.13703/j.0255-2930.2017.10.017.
To explore the effect of electroacupuncture(EA) preconditioning on cerebral infarct volume and the contents of TNF-α, IL-10 in serum of rats with cerebral ischemia-reperfusion injury.
Thirty-six rats were randomly divided into a sham operation group, a model group and an EA preconditioning group, 12 rats in each group, which were further divided into 12 h and 24 h after reperfusion subgroups, 6 rats in each one. EA was used before model establishment for 2 weeks in the EA preconditioning group. The model of cerebral ischemia-reperfusion injury in rats was established with modified Longa suture method. 12 h and 24 h after reperfusion, the degree of neurological deficit was assessed by the modified behavioral scoring scale; the cerebral infarct volume was measured by TTC method and the contents of TNF-α, IL-10 in serum were detected by ELISA method.
Compared with the model group, the neurological severity scores in the EA preconditioning group significantly reduced 12 h and 24 h after reperfusion (both <0.05), the cerebral infarct volume in the EA preconditioning group significantly reduced 12 h and 24 h after reperfusion (both <0.05). Compared with the sham operation group, the serum TNF-α, IL-10 contents in the model group increased 12 h and 24 h after reperfusion (both <0.05). Compared with the model group, the serum TNF-α content reduced, while the serum IL-10 content increased in the EA preconditioning group 12 h after reperfusion (both <0.05). Compared with the model group, the serum TNF-α, IL-10 contents reduced in the EA preconditioning group 24 h after reperfusion (both <0.05).
EA preconditioning can improve neurological deficit, reduce cerebral infarct volume after cerebral ischemia-reperfusion injury in rats. The mechanism may be related to the regulation of EA on the dynamic balance between pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in peripheral blood of cerebral ischemia-reperfusion injury in acute phase, thus alleviate acute cerebral ischemia-reperfusion inflammatory response.
探讨电针预处理对脑缺血再灌注损伤大鼠脑梗死体积及血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）含量的影响。
将36只大鼠随机分为假手术组、模型组和电针预处理组，每组12只，每组再分为再灌注后12 h和24 h亚组，各亚组6只。电针预处理组在造模前2周采用电针干预。采用改良的Longa线栓法制备大鼠脑缺血再灌注损伤模型。再灌注12 h和24 h后，采用改良的行为评分量表评估神经功能缺损程度；采用TTC法测量脑梗死体积，采用ELISA法检测血清中TNF-α、IL-10的含量。
与模型组比较，电针预处理组再灌注12 h和24 h时神经功能严重程度评分显著降低（均P<0.05），脑梗死体积显著减小（均P<0.05）。与假手术组比较，模型组再灌注12 h和24 h时血清TNF-α、IL-10含量升高（均P<0.05）。与模型组比较，电针预处理组再灌注12 h时血清TNF-α含量降低，IL-10含量升高（均P<0.05）。与模型组比较，电针预处理组再灌注24 h时血清TNF-α、IL-10含量降低（均P<0.05）。
电针预处理可改善大鼠脑缺血再灌注损伤后的神经功能缺损，减小脑梗死体积。其机制可能与电针调节急性期脑缺血再灌注损伤大鼠外周血中促炎细胞因子TNF-α与抗炎细胞因子IL-10的动态平衡有关，从而减轻急性脑缺血再灌注炎症反应。
