De Iorio P, Midulla C, Pisani T, Valli C, Grillo L, Di Cocco B, Nofroni I, Vecchione A
Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Viale Regina Elena n.324, 00162, Rome, Italy.
Anticancer Res. 2001 Mar-Apr;21(2B):1395-9.
The prognostic variability in breast cancer patients prompted the authors to investigate specific biological markers for the identification of high-risk breast cancer groups. In the present study, attention was focused on the interaction between tumor cells and the extracellular matrix, an important requisite in the metastatic process.
Fifty-six primary breast carcinoma specimens obtained by mastectomy or quadrantectomy plus axillary dissection were examined with immunohistochemistry, for the determination of laminin, collagen type IV and hormone receptor expression and with static cytometry, for the determination of the DNA content.
Laminin and collagen type IV expression was observed on the membrane and in the cytoplasm of neoplastic cells. Laminin and collagen type IV were present, respectively, in 85.4% and 73.8% of the cases which showed recurrence.
The results of this study have shown that high expression of laminin and collagen type IV may have a value in the prognosis of disease free survival and may be linked to other classical clinical, histological and biological parameters in the evaluation of breast cancer patients.
乳腺癌患者预后的变异性促使作者研究用于识别高危乳腺癌群体的特定生物标志物。在本研究中，重点关注肿瘤细胞与细胞外基质之间的相互作用，这是转移过程中的一个重要条件。
对通过乳房切除术或象限切除术加腋窝清扫术获得的56例原发性乳腺癌标本进行免疫组织化学检查，以测定层粘连蛋白、IV型胶原和激素受体表达，并进行静态细胞计数，以测定DNA含量。
在肿瘤细胞的细胞膜和细胞质中观察到层粘连蛋白和IV型胶原表达。层粘连蛋白和IV型胶原分别存在于85.4%和73.8%出现复发的病例中。
本研究结果表明，层粘连蛋白和IV型胶原的高表达可能对无病生存期的预后有价值，并且在评估乳腺癌患者时可能与其他经典的临床、组织学和生物学参数相关。
