Uchida Y, Shiba H, Komatsuzawa H, Takemoto T, Sakata M, Fujita T, Kawaguchi H, Sugai M, Kurihara H
Department of Periodontology and Endodontology, Hiroshima University Faculty of Dentistry, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan, 734-8553.
Cytokine. 2001 May 7;14(3):152-61. doi: 10.1006/cyto.2001.0863.
The interaction between epithelial cells and microorganisms is the most important step in bacterial infections. Epithelial cells in response to exposure to pathogenic bacteria produce cytokines that initiate inflammation. However, little is known about the cytokine response of gingival epithelial cells to periodontopathogenic bacteria. Actinobacillus actinomycetemcomitans is thought to play a significant role in the initiation of periodontitis because of its bacteriological characteristics. In the present study, we investigated the cytokine induction by human gingival epithelial cells (HGEC) following exposure to A. actinomycetemcomitans in comparison with human gingival fibroblasts (HGF) in culture. Northern blot analysis showed that mRNAs of interleukin 1beta (IL-1beta) and IL-8, but not IL-6, in HGEC were induced in response to A. actinomycetemcomitans. Secretion of IL-8 by HGEC was also increased following A. actinomycetemcomitans challenge, whereas production of IL-1beta could not be detected. The levels of IL-8 and its mRNA were increased depending on the concentration of A. actinomycetemcomitans. The co-culture with HGF and A. actinomycetemcomitans resulted in an increase in the levels of IL-6 and IL-8 mRNA in HGF. However, HGF exposed to A. actinomycetemcomitans, showed no expression of IL-1beta mRNA. These findings demonstrated that HGEC and HGF stimulated with A. actinomycetemcomitans have different profiles in cytokine mRNA expression. Furthermore, A. actinomycetemcomitans may play an important role in amplifying the local immune response and in initiating inflammatory reaction through release of IL-8 from gingival epithelial cells.
上皮细胞与微生物之间的相互作用是细菌感染中最重要的步骤。上皮细胞在接触病原菌后会产生引发炎症的细胞因子。然而，关于牙龈上皮细胞对牙周病原菌的细胞因子反应却知之甚少。由于其细菌学特性，伴放线放线杆菌被认为在牙周炎的起始过程中起重要作用。在本研究中，我们将培养的人牙龈上皮细胞（HGEC）与人类牙龈成纤维细胞（HGF）相比较，研究了它们在接触伴放线放线杆菌后细胞因子的诱导情况。Northern印迹分析表明，HGEC中的白细胞介素1β（IL-1β）和IL-8的mRNA会因接触伴放线放线杆菌而被诱导，但IL-6的mRNA不会。在伴放线放线杆菌攻击后，HGEC分泌的IL-8也增加了，而未检测到IL-1β的产生。IL-8及其mRNA的水平会根据伴放线放线杆菌的浓度而增加。HGF与伴放线放线杆菌共培养导致HGF中IL-6和IL-8 mRNA水平增加。然而，接触伴放线放线杆菌的HGF未显示IL-1β mRNA的表达。这些发现表明，受伴放线放线杆菌刺激的HGEC和HGF在细胞因子mRNA表达方面具有不同的特征。此外，伴放线放线杆菌可能通过从牙龈上皮细胞释放IL-8在放大局部免疫反应和引发炎症反应中起重要作用。
