黄曲霉毒素B1产生的单体核糖体的特性分析

Characterization of monosomes produced by aflatoxin B1.

作者信息

Hayes L C, Plapp F V, Tilzer L L, Chiga M

出版信息

Chem Biol Interact. 1975 May;10(5):343-7. doi: 10.1016/0009-2797(75)90056-3.

Abstract

A single injection of 1.5 mg aflatoxin B1 per kg body weight produced approx. 70% disaggregation of rat liver polysomes into monosomes within 18 h. Isolated monosomes dissociated into 40 S subunits during centrifugation in linear sucrose gradients containing 0.3 M KCI. The 4 S to 5 S molar RNA ratio of the monosomes was calculated to be 0.6, indicating 0.6 tRNA and/or aminoacyl tRNA molecule per ribosome; no peptidyl tRNA was present. These results suggest that a single injection of affatoxin B1 produces monosomes which resemble runoff ribosomes.

摘要

每千克体重单次注射1.5毫克黄曲霉毒素B1，在18小时内可使大鼠肝脏多核糖体大约70%解聚为单核糖体。在含有0.3M氯化钾的线性蔗糖梯度中离心时，分离出的单核糖体解离为40S亚基。单核糖体的4S至5S摩尔RNA比率经计算为0.6，表明每个核糖体有0.6个转运RNA和/或氨酰转运RNA分子；不存在肽基转运RNA。这些结果表明，单次注射黄曲霉毒素B1可产生类似于延伸后核糖体的单核糖体。