游离核糖体与膜结合核糖体。II. 兔网织红细胞游离核糖体与膜结合核糖体蛋白质的二维凝胶电泳
Free and membrane-bound ribosomes. II. Two-dimensional gel electrophoresis of proteins from free and membrane-bound rabbit reticulocyte ribosomes.
作者信息
Fehlmann M, Bellemare G, Godin C
出版信息
Biochim Biophys Acta. 1975 Jan 6;378(1):119-24. doi: 10.1016/0005-2787(75)90142-2.
Free and membrane-bound polysomes were isolated from rabbit reticulocytes. The membrane-bound polysomes were liberated form the membrane with deoxycholate. Monosomes were prepared from the two types of polysomes by incubation with puromycin. The ribosomal proteins were extracted and analyzed by two-dimensional gel electrophoresis. Two proteins of the large subunit, L11 and L17 present in the free monosomes were not found in the membrane-bound monosomes. On the other hand, four additional spots were found in the protein pattern of the membrane-bound monosomes.
从兔网织红细胞中分离出游离的和结合于膜上的多核糖体。用脱氧胆酸盐使结合于膜上的多核糖体从膜上释放出来。通过与嘌呤霉素一起温育，从这两种类型的多核糖体中制备出单体核糖体。提取核糖体蛋白并用双向凝胶电泳进行分析。在游离单体核糖体中存在的两个大亚基蛋白L11和L17，在结合于膜上的单体核糖体中未被发现。另一方面，在结合于膜上的单体核糖体的蛋白质图谱中发现了另外四个斑点。
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