Armstead I P, Bollard A, Morgan W G, Harper J A, King I P, Jones R N, Forster J W, Hayward M D, Thomas H M
Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion, UK.
Chromosoma. 2001 Apr;110(1):52-7. doi: 10.1007/s004120000122.
Molecular marker analysis and genomic in situ hybridisation (GISH) were used to examine the process of chromosome segment introgression in BC2 diploid hybrids (2n=2x=14) between Lolium perenne and Festuca pratensis. Two genotypes having what appeared to be the same, single, introgressed chromosome segment of F. pratensis in the L. perenne background were crossed with diploid L. perenne to produce a recombinant series for the introgressed region. Physical and genetic analysis of this series showed that, while recombination seemed to be possible at all points along the chromosome arm, the rate of recombination varied depending on relative position: more recombination was detected in the interstitial region as compared with the centromeric or telomeric regions. The implications of these results for the use of GISH and molecular marker analysis in the measurement of linkage drag in backcross breeding programmes is discussed.
利用分子标记分析和基因组原位杂交（GISH）技术，研究了黑麦草（Lolium perenne）和草地羊茅（Festuca pratensis）之间BC2二倍体杂种（2n = 2x = 14）中染色体片段渐渗的过程。将两个在黑麦草背景下具有似乎相同的、单一的草地羊茅渐渗染色体片段的基因型与二倍体黑麦草杂交，以产生渐渗区域的重组系列。对该系列的物理和遗传分析表明，虽然沿着染色体臂的所有点似乎都可能发生重组，但重组率因相对位置而异：与着丝粒或端粒区域相比，在中间区域检测到更多的重组。讨论了这些结果对于在回交育种计划中利用GISH和分子标记分析来测量连锁累赘的意义。
