检测耐甲氧西林金黄色葡萄球菌的改进方法。
Improved methods for detection of methicillin-resistant Staphylococcus aureus.
作者信息
Rohrer S, Tschierske M, Zbinden R, Berger-Bächi B
机构信息
Department of Medical Microbiology, University of Zürich, Switzerland.
出版信息
Eur J Clin Microbiol Infect Dis. 2001 Apr;20(4):267-70. doi: 10.1007/pl00011263.
In order to assess the performance of two detection methods, a set of 93 recent clinical isolates of Staphylococcus aureus, including a large number of strains that demonstrated low-level methicillin-resistance were evaluated using the MRSA-Screen (Denka Seiken, Japan), a commercial latex agglutination test to detect penicillin-binding protein 2' (PBP2'), and a polymerase chain reaction assay using the LightCycler Instrument (Roche Diagnostics, Switzerland). The results show that the latex agglutination test is highly sensitive if performed after induction by cefoxitin. Inconclusive results can be rapidly confirmed on the same day by real-time polymerase chain reaction used to detect mecA and femA genes.
为了评估两种检测方法的性能，使用MRSA-Screen（日本Denka Seiken公司）对一组93株近期临床分离的金黄色葡萄球菌进行了评估，该检测方法是一种用于检测青霉素结合蛋白2'（PBP2'）的商业乳胶凝集试验，同时还使用了罗氏诊断公司（瑞士）的LightCycler仪器进行聚合酶链反应分析。这些临床分离株包括大量表现出低水平耐甲氧西林的菌株。结果表明，如果在头孢西丁诱导后进行乳胶凝集试验，则该试验具有高度敏感性。通过用于检测mecA和femA基因的实时聚合酶链反应，可以在同一天快速确认不确定的结果。
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