Louie L, Matsumura S O, Choi E, Louie M, Simor A E
Department of Microbiology, SD Laboratory Services, Sunnybrook and Women's College Health Sciences Centre, and the University of Toronto, Toronto, Ontario M4N 3M5, Canada.
J Clin Microbiol. 2000 Jun;38(6):2170-3. doi: 10.1128/JCM.38.6.2170-2173.2000.
The probe-based Velogene Rapid MRSA Identification Assay (ID Biomedical Corp., Vancouver, British Columbia, Canada) and the latex agglutination MRSA-Screen (Denka Seiken Co., Tokyo, Japan) were evaluated for their ability to identify methicillin-resistant Staphylococcus aureus (MRSA) and to distinguish strains of MRSA from borderline oxacillin-resistant S. aureus (BORSA; mecA-negative, oxacillin MICs of 2 to 8 microgram/ml). The Velogene is a 90-min assay using a chimeric probe to detect the mecA gene. MRSA-Screen is a 15-min latex agglutination test with penicillin-binding protein 2a antibody-sensitized latex particles. We compared these assays with the BBL Crystal MRSA ID System (Becton Dickinson, Cockeysville, Md.) and with PCR for mecA gene detection. A total of 397 clinical isolates of S. aureus were tested, consisting of 164 methicillin-susceptible strains, 197 MRSA strains, and 37 BORSA strains. All assays performed well for the identification of MRSA with sensitivities and specificities for Velogene, MRSA-Screen, and BBL Crystal MRSA ID of 98.5 and 100%, 98.5 and 100%, and 98.5 and 98%, respectively. Three MRSA strains were not correctly identified by each of the Velogene and MRSA-Screen assays, but repeat testing with a larger inoculum resolved the discrepancies. The BBL Crystal MRSA ID test misclassified four BORSA strains as MRSA. Both the Velogene and the MRSA-Screen assays are easy to perform, can accurately differentiate BORSA isolates from MRSA isolates, and provide a rapid alternative for the detection of methicillin resistance in S. aureus in clinical laboratories, especially when mecA PCR gene detection is unavailable.
对基于探针的Velogene快速耐甲氧西林金黄色葡萄球菌鉴定检测法（ID生物医学公司，加拿大不列颠哥伦比亚省温哥华）和乳胶凝集法MRSA-Screen（日本东京电和化成工业株式会社）进行了评估，以确定它们鉴定耐甲氧西林金黄色葡萄球菌（MRSA）以及区分MRSA菌株与临界耐苯唑西林金黄色葡萄球菌（BORSA；mecA阴性，苯唑西林最低抑菌浓度为2至8微克/毫升）的能力。Velogene检测法是一种耗时90分钟的检测法，使用嵌合探针检测mecA基因。MRSA-Screen是一种耗时15分钟的乳胶凝集试验，采用青霉素结合蛋白2a抗体致敏乳胶颗粒。我们将这些检测法与BBL Crystal MRSA ID系统（美国马里兰州科基斯维尔的BD公司）以及用于检测mecA基因的聚合酶链反应（PCR）进行了比较。共检测了397株金黄色葡萄球菌临床分离株，其中包括164株甲氧西林敏感菌株、197株MRSA菌株和37株BORSA菌株。所有检测法在鉴定MRSA方面表现良好，Velogene、MRSA-Screen和BBL Crystal MRSA ID的敏感性和特异性分别为98.5%和100%、98.5%和100%、98.5%和98%。有3株MRSA菌株未被Velogene和MRSA-Screen检测法正确鉴定，但加大接种量重复检测解决了这些差异。BBL Crystal MRSA ID检测将4株BORSA菌株误分类为MRSA。Velogene和MRSA-Screen检测法都易于操作，能够准确区分BORSA分离株与MRSA分离株，为临床实验室检测金黄色葡萄球菌中的甲氧西林耐药性提供了一种快速替代方法，尤其是在无法进行mecA PCR基因检测时。
