Gouÿ de Bellocq J, Ferté H, Depaquit J, Justine J L, Tillier A, Durette-Desset M C
Laboratoire de Biologie Parasitaire, Protistologie, et Helminthologie, Muséum National d'Histoire Naturelle, 61 Rue Buffon, Paris Cedex 05, 75231, France.
Mol Phylogenet Evol. 2001 Jun;19(3):430-42. doi: 10.1006/mpev.2001.0925.
We produced a molecular phylogeny of species within the order Strongylida (bursate nematodes) using the D1 and D2 domains of 28S rDNA, with 23 new sequences for each domain. A first analysis using Caenorhabditis elegans as an outgroup produced a tree with low resolution in which three taxa (Dictyocaulus filaria, Dictyocaulus noerneri, and Metastrongylus pudendotectus) showed highly divergent sequences. In a second analysis, these three species and C. elegans were removed and an Ancylostomatina, Bunostomum trigonocephalum, was chosen (on the basis of previous morphological analyses) as the outgroup for an analysis of the phylogenetic relationships between and within the Strongylina (strongyles) and Trichostrongylina (trichostrongyles). A very robust tree was obtained. The Trichostrongylina were monophyletic, but the Strongylina were paraphyletic, though this requires confirmation. Within the Trichostrongylina, the three superfamilies defined from morphological characters are confirmed, with the Trichostrongyloidea sister group to a clade including the Molineoidea and Heligmosomoidea. Within the Trichostrongyloidea, the Cooperiidae, Trichostrongylidae, and Haemonchidae were polytomous, the Haemonchinae were monophyletic, but the Ostertagiinae were paraphyletic. The sister-group relationships between Molineoidea and Heligmosomoidea were unsuspected from previous morphological analysis. No unequivocal morphological synapomorphy could be found for the grouping Molineoidea + Heligmosomoidea, but none was found which contradicted it.
我们利用28S rDNA的D1和D2结构域构建了圆线目（有囊线虫）内各物种的分子系统发育树，每个结构域有23条新序列。首次分析以秀丽隐杆线虫作为外类群，得到的树分辨率较低，其中三个分类单元（丝状网尾线虫、诺氏网尾线虫和阴户突圆线吸虫）显示出高度分歧的序列。在第二次分析中，移除了这三个物种和秀丽隐杆线虫，并根据先前的形态学分析选择了钩口亚目（Bunostomum trigonocephalum）作为外类群，用于分析圆线亚目（圆线虫）和毛圆亚目（毛圆线虫）之间及内部的系统发育关系。得到了一棵非常稳健的树。毛圆亚目是单系的，但圆线亚目是并系的，不过这一点需要进一步确认。在毛圆亚目内，根据形态特征定义的三个超科得到了确认，毛圆超科是包括莫尼茨超科和细颈线虫超科在内的一个进化枝姐妹群。在毛圆超科内，库珀科、毛圆科和血矛科是多歧的，血矛亚科是单系的，但奥斯特塔吉亚科是并系的。莫尼茨超科和细颈线虫超科之间的姐妹群关系在先前的形态学分析中未被怀疑。对于莫尼茨超科+细颈线虫超科的分组，未发现明确的形态共衍征，但也没有发现与之矛盾的特征。
