Halford S, Freedman M S, Bellingham J, Inglis S L, Poopalasundaram S, Soni B G, Foster R G, Hunt D M
Department of Molecular Genetics, Institute of Ophthalmology, University College London, 11-43 Bath Street, London, EC1V 9EL, United Kingdom.
Genomics. 2001 Mar 1;72(2):203-8. doi: 10.1006/geno.2001.6469.
As part of an ongoing search to identify novel mammalian photopigments that may mediate nonvisual tasks such as circadian entrainment and acute suppression of pineal melatonin levels, a number of recently cloned nonvisual opsin sequences were used to search dbEST. panopsin (OPN3) was one of the clones identified using this approach. Expression analysis detects two transcripts of approximately 2.1 and 2.5 kb, in a wide range of tissues including brain, liver, and retina, which encode a predicted protein of 403 amino acids. The gene was localized to the region of chromosome 1q43 also encompassing the kynurenine monooxygenase (KMO) and choroideremia-like Rab escort protein 2 (CHML) genes. KMO and panopsin overlap at their 3' ends but are transcribed in opposite directions. CHML, an intronless gene, lies in intron 1 of panopsin.
作为正在进行的一项研究的一部分，旨在鉴定可能介导诸如昼夜节律调节和松果体褪黑素水平急性抑制等非视觉任务的新型哺乳动物光色素，一些最近克隆的非视觉视蛋白序列被用于搜索dbEST。泛视蛋白（OPN3）是用这种方法鉴定出的克隆之一。表达分析在包括脑、肝和视网膜在内的多种组织中检测到两条分别约为2.1 kb和2.5 kb的转录本，它们编码一种预测的含403个氨基酸的蛋白质。该基因定位于1号染色体q43区域，该区域还包含犬尿氨酸单加氧酶（KMO）和类脉络膜视网膜病变Rab护送蛋白2（CHML）基因。KMO和泛视蛋白在其3'端重叠，但转录方向相反。CHML是一个无内含子基因，位于泛视蛋白的第1内含子中。
