González S, Caballero E, Silva R, Pajón R
División de Vacunas, Centro de Ingeniería Genética y Biotecnología, Ciudad Havana CP, 10600, Cuba.
Biochem Biophys Res Commun. 2001 Mar 30;282(2):376-9. doi: 10.1006/bbrc.2001.4581.
Recently, we have successfully employed the meningococcal P64k protein as a carrier for weak immunogens. Here, we study if presensitization with it can affect the murine antibody response against the hapten chemically coupled to P64k. We found that priming with 10 microg of P64k did not induce epitope-specific suppression against two out of three synthetic peptides, from viral proteins, conjugated to this carrier. Depending on the anti-carrier antibody titers elicited in the presensitized mice, we observed or not a suppressed immune response against the third peptide. Presensitization with 100 microg of P64k resulted in epitope-specific suppression when lower doses of conjugate were administered. In summary, as described for other protein carriers, P64k could induce epitope-specific suppression in mice, but it depends on the hapten and the extent of carrier-specific immunity. Furthermore, this suppression can be overcome by increasing the amount of conjugate administered per dose in the presensitized animals.
最近，我们已成功将脑膜炎球菌P64k蛋白用作弱免疫原的载体。在此，我们研究用它进行预致敏是否会影响小鼠针对化学偶联到P64k的半抗原的抗体反应。我们发现，用10微克P64k进行初次免疫不会诱导针对与该载体偶联的三种病毒蛋白合成肽中两种的表位特异性抑制。根据预致敏小鼠中引发的抗载体抗体滴度，我们观察到或未观察到针对第三种肽的免疫反应受到抑制。当给予较低剂量的偶联物时，用100微克P64k进行预致敏会导致表位特异性抑制。总之，正如针对其他蛋白质载体所描述的那样，P64k可在小鼠中诱导表位特异性抑制，但这取决于半抗原和载体特异性免疫的程度。此外，这种抑制可通过在预致敏动物中增加每剂量给予的偶联物量来克服。
