González Sonia, Nazábal Consuelo, Rao Kanury V S, Reyes Osvaldo, Garay Hilda E, Caballero Evelin, Alvarez-Obregón Julio C, Sardiñas Gretel, Silva Ricardo
Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba.
FEMS Immunol Med Microbiol. 2004 Oct 1;42(2):233-9. doi: 10.1016/j.femsim.2004.05.007.
The P64k protein of Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunized with the recombinant antigen provided inguinal lymph node cells (LNC) that proliferated in the presence of P64k in a dose-dependent manner. Proliferating cells secreted IL-4 while the concentration of IL-12 remained unaltered in the culture supernatant. By testing a panel of 59 overlapping synthetic peptides spanning the entire sequence of the antigen a T-cell determinant was localized. Prime-boost and lymphoproliferation experiments, conducted with highly purified synthetic peptides, confirmed that the segment including amino acids 470-485 comprises a T-cell epitope within the P64k molecule.
脑膜炎奈瑟菌的P64k蛋白已被报道为弱免疫原的免疫载体。本研究旨在表征致敏小鼠产生的T细胞反应，并确定该分子内的T辅助细胞表位。用重组抗原皮下免疫的BALB/c小鼠提供了腹股沟淋巴结细胞（LNC），这些细胞在P64k存在的情况下以剂量依赖方式增殖。增殖细胞分泌IL-4，而培养上清液中IL-12的浓度保持不变。通过测试一组覆盖该抗原整个序列的59个重叠合成肽，定位了一个T细胞决定簇。用高度纯化的合成肽进行的初免-加强免疫和淋巴细胞增殖实验证实，包括氨基酸470-485的片段在P64k分子内构成一个T细胞表位。
