Brewer W E, Galipo R C, Sellers K W, Morgan S L
Toxicology Department, South Carolina Law Enforcement Division, Columbia 29210, USA.
Anal Chem. 2001 Jun 1;73(11):2371-6. doi: 10.1021/ac000871r.
Supercritical fluid extraction (SFE) using CO2 modified with methanol (10%) was employed for the extraction of cocaine from human hair. Extraction conditions were developed by using designed experiments to characterize the effects of pressure, temperature, and percent methanol on the recovery of cocaine from hair. When compared to the conventional acid hydrolysis method for hair analysis, SFE was faster and gave higher recoveries. Amounts of cocaine, benzoylecgonine (cocaine metabolite), codeine, and morphine in a hair standard reference material were determined by SFE-gas chromatography/ mass spectrometry (GC/MS) and found to be in agreement with reported levels. Analyses of hair from forensic case studies are also reported.
采用用甲醇（10%）改性的二氧化碳进行超临界流体萃取（SFE），从人发中提取可卡因。通过设计实验来确定萃取条件，以表征压力、温度和甲醇百分比对从头发中回收可卡因的影响。与传统的头发分析酸水解方法相比，SFE速度更快且回收率更高。通过SFE-气相色谱/质谱联用（GC/MS）测定了头发标准参考物质中可卡因、苯甲酰爱康宁（可卡因代谢物）、可待因和吗啡的含量，结果与报道水平一致。还报告了法医案例研究中的头发分析情况。
