超临界流体及其在生物技术和相关领域的应用。

Supercritical fluids and their applications in biotechnology and related areas.

作者信息

Williams John R, Clifford Anthony A, al-Saidi Salim H R

机构信息

Department of Chemistry, College of Science, Sultan Qaboos University, PO Box 36, Al-Khod 123, Sultanate of Oman.

出版信息

Mol Biotechnol. 2002 Nov;22(3):263-86. doi: 10.1385/MB:22:3:263.

DOI:10.1385/MB:22:3:263

PMID:12448881

Abstract

This article serves as an overview, introducing the currently popular area of supercritical fluids (SFs) and their uses in biotechnology and related areas. It covers the fundamentals of supercritical science and moves on to the biotechnological and associated applications of these fluids. Subject areas covered include pure substances as supercritical fluids, the properties of supercritical fluids, organic cosolvents, solubility, and the following applications: extraction, chromatography, reactions, particle production, deposition, and the drying of biological specimens. Within each application, and where possible, the basic principles of the technique are given, as well as a description of the history, instrumentation, methodology, uses, problems encountered, and advantages over the traditional, nonsupercritical methods.

摘要

本文作为概述，介绍了超临界流体（SFs）当前热门的领域及其在生物技术和相关领域的应用。它涵盖了超临界科学的基础，并进而探讨了这些流体在生物技术及相关方面的应用。所涵盖的主题领域包括作为超临界流体的纯物质、超临界流体的性质、有机共溶剂、溶解度，以及以下应用：萃取、色谱法、反应、颗粒生产、沉积和生物标本干燥。在每个应用中，尽可能给出该技术的基本原理，以及对其历史、仪器设备、方法、用途、遇到的问题以及相对于传统非超临界方法的优势的描述。