一种用于反刍兽无形体生长的化学成分明确的培养基。

A chemically defined medium for the growth of Cowdria ruminantium.

作者信息

Zweygarth E, Josemans A I

机构信息

Parasitology Division, Onderstepoort Veterinary Institute, South Africa.

出版信息

Onderstepoort J Vet Res. 2001 Mar;68(1):37-40.

PMID:11403428

Abstract

Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 (DME/F-12) containing various supplements. Medium SFMC-23 and SFMC-36 supported the long-term growth of the Welgevonden stock of C. ruminantium for a total of 55 and 28 passages, respectively, with regular passage intervals of 3 days. Using SFMC-23, split ratios varied from 5-10, depending on which host cell line was used. Other stocks of C. ruminantium (Sankat, Blaauwkrantz, Senegal) were successfully propagated for a test period of ten passages.

摘要

为反刍动物心水病病原体——反刍兽考德里氏体（Cowdria ruminantium）的体外培养设计了化学成分明确的培养基，分别称为SFMC - 23和SFMC - 36。两种培养基均基于含有各种补充剂的杜尔贝科改良伊格尔培养基营养混合物Ham F - 12（DME/F - 12）。培养基SFMC - 23和SFMC - 36分别支持反刍兽考德里氏体韦尔格冯登株系的长期生长，传代次数分别为55次和28次，传代间隔为3天。使用SFMC - 23时，传代比例因使用的宿主细胞系而异，范围为5至10。反刍兽考德里氏体的其他株系（桑卡特、布劳克兰茨、塞内加尔）在为期十代的测试期内成功传代。