无血清培养条件下反刍动物考德里氏体的体外分离与培养

In vitro isolation and cultivation of Cowdria ruminantium under serum-free culture conditions.

作者信息

Zweygarth E, Vogel S W, Josemans A I, Horn E

机构信息

Onderstepoort Veterinary Institute, Protozoology Division, South Africa.

出版信息

Res Vet Sci. 1997 Sep-Oct;63(2):161-4. doi: 10.1016/s0034-5288(97)90011-4.

DOI:10.1016/s0034-5288(97)90011-4

PMID:9429251

Abstract

Three stocks of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, were propagated in bovine endothelial cells in a serum-free culture medium. The Vosloo, Welgevonden and Senegal stocks were propagated for a period of more than 203, 134, and 43 days, respectively. Two of the C ruminantium stocks (Vosloo and Senegal) were also successfully initiated under serum-free culture conditions. The serum-free medium consisted of a modified HL-1 medium. The Senegal stock was successfully propagated in Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 per cent fetal bovine serum.

摘要

反刍动物心水病的病原体——反刍兽考德里氏体的三株菌在无血清培养基中的牛内皮细胞中进行传代培养。Vosloo、Welgevonden和塞内加尔菌株分别传代培养了203天以上、134天和43天。两株反刍兽考德里氏体菌株（Vosloo和塞内加尔）也在无血清培养条件下成功传代。无血清培养基由改良的HL-1培养基组成。塞内加尔菌株在含有10%胎牛血清的杜氏改良伊格尔培养基营养混合物Ham F-12中成功传代。