Zweygarth E, Josemans A I
Parasitology Division, Onderstepoort Veterinary Institute Private Bag X05, Onderstepoort, 0110 South Africa.
Onderstepoort J Vet Res. 2003 Jun;70(2):165-8.
The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for reliable growth of E. ruminantium. Growth of the Welgevonden stock in BHK and CHO-K1 cells could lead to the development of suspension cultures suitable for the mass production of E. ruminantium for an inactivated elementary body vaccine.
反刍动物埃立克体(心水病的病原体)的韦尔格冯登毒株在幼仓鼠肾(BHK)细胞、中国仓鼠卵巢(CHO-K1)细胞和马迪达比牛肾(MDBK)细胞中进行传代培养。这些培养物需要在培养基中添加放线菌酮,以确保反刍动物埃立克体可靠生长。韦尔格冯登毒株在BHK和CHO-K1细胞中的生长可促使悬浮培养物的形成,适合大规模生产用于灭活原体疫苗的反刍动物埃立克体。