用于大规模蛋白质相互作用图谱绘制的高通量酵母双杂交分析。

High-throughput yeast two-hybrid assays for large-scale protein interaction mapping.

作者信息

Walhout A J, Vidal M

机构信息

Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

出版信息

Methods. 2001 Jul;24(3):297-306. doi: 10.1006/meth.2001.1190.

DOI:10.1006/meth.2001.1190

PMID:11403578

Abstract

Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system.

摘要

蛋白质-蛋白质相互作用在许多生物学过程中发挥着基本作用。因此，蛋白质相互作用图谱绘制正成为一种成熟的功能基因组学方法，用于为迄今尚未表征的预测蛋白质生成功能注释。酵母双杂交系统是目前最标准化的蛋白质相互作用图谱绘制技术之一。在此，我们描述了一种基于Gal4的半自动化、高通量酵母双杂交系统的实验方案。