Janssen D B, Oppentocht J E, Poelarends G J
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL-9747 AG, Groningen, The Netherlands.
Curr Opin Biotechnol. 2001 Jun;12(3):254-8. doi: 10.1016/s0958-1669(00)00208-1.
Novel dehalogenases have been identified recently in various bacteria that utilise halogenated substrates. X-ray studies and sequence analysis have revealed insight into the molecular mechanisms of hydrolytic dehalogenases. Furthermore, genetic and biochemical studies have indicated that reductive dehalogenases are extra-cytoplasmic corrinoid-containing iron-sulphur proteins. Sequence analysis and mutagenesis studies indicate that several dehalogenases are homologous to enzymes that carry out transformations on non-halogenated substrates.
最近在各种利用卤代底物的细菌中发现了新型脱卤酶。X射线研究和序列分析揭示了对水解脱卤酶分子机制的深入了解。此外，遗传学和生物化学研究表明，还原脱卤酶是含胞外类咕啉的铁硫蛋白。序列分析和诱变研究表明，几种脱卤酶与对非卤代底物进行转化的酶同源。
